Б.Одмаа¹, М.Туул², Д.Амгаланбаатар³

¹ЭМШУИС-Эрүүл Мэндийн Технологийн Сургууль, ²Анагаах ухааны хүрээлэн, ³ЭМШУИС-Биоанагаахын Сургууль

Bacground: Liver cancer is the 5th most common cancer worldwide with 500,000 cases diagnosed per year. It is a disease with a high death rate (14000-15000 per year). By the last news of national center of health development, liver cancer is first most common cancer in our country. 

Goal: To study and to compare volumetric modeling of hepatocyte’s cytoplasm, nucleus and stereometric indices in condition of comparatively healthy, acute intoxication, chronic inflammation, cancerous condition and clear cell tumor, and to explain by non linear theory.

Materials and Methods: It was prepared sections for histometric materials. Linear measurements of hepatocytes and nucleus were carried out by computer microscope ‘Leica’ with program Diskus 3.2 version from GermanOn linear measurement in condition of comparatively healthy, acute intoxication, chronic inflammation, cancerous condition and clear cell tumor were processed by mathematic modeling. 

Results: In comparatively healthy condition the volume of the hepatocyte’s cytoplasm was determined 2140.73±19.97 mkm3, the volume of the hepatocyte’s nucleus was 295.19±2.60 mkm3, ration between hepatocyte’s cytoplasm and nucleus was 7:1 (P<0.001). In condition of acute intoxication the volume of the hepatocyte’s cytoplasm was determined 4281.36±77.83 mkm3, the volume of the hepatocyte’s nucleus was 895.00±13.42 mkm3, ratio between hepatocyte’s cytoplasm and nucleus was 5:1 (P<0.001). In condition of chronic inflammation the volume of the hepatocyte’s cytoplasm was determined 4887.84±75.72 mkm3, the volume of the hepatocyte’s nucleus was 888.65±12.46 mkm3, ration between hepatocyte’s cytoplasm and nucleus was 5:1 (P<0.001). In cancerous condition the volume of the hepatocyte’s cytoplasm was determined 3852.63±116.06 mkm3, the volume of the hepatocyte’s nucleus was 463.09±12.95 mkm3, ratio between hepatocyte’s cytoplasm and nucleus was 8:1 (P<0.001). In clear cell tumor the volume of the hepatocyte’s cytoplasm was determined 15062.69±348.41 mkm3, the volume of the hepatocyte’s nucleus was 801.05±22.56 mkm3, ratio between hepatocyte’s cytoplasm and nucleus was 19:1 (P<0.001). 

Conclusions:

1. We have determined volume of hepatocyte’s volume and nucleus, ratio between cytoplasm and nucleus in condition of comparatively healthy, acute intoxication, chronic inflammation, cancerous and clear cell tumor.
2. Volume of hepatocyte’s cytoplasm was increased 2.28 times, volume of hepotocyte’s nucleus was increased 3.01 times in condition of chronic inflammation and  volume of hepatocyte’s cytoplasm was increased 1.99 times, volume of hepotocyte’s nucleus was increased 3.03 times in condition of acute intoxication. Also volume of hepatocyte’s cytoplasm was increased 1.79 times, volume of hepotocyte’s nucleus was increased 1.5 times in cancerous condition and volume of hepatocyte’s cytoplasm was increased 7.03 times, volume of hepotocyte’s nucleus was increased 2.7 times in condition of clear cell tumor.
3. By the comparison between volumes of hepatocyte’s cytoplasm and nucleus in condition of comparatively healthy, acute intoxication, chronic inflammation, cancerous was determined medium direct correlation rather by the comparison between volumes of hepatocyte’s cytoplasm and nucleus in condition of clear cell tumor wasn’t determined correlation.

Pp.11-16 , Tables 6, References 21