Дорно дахины уламжлалт анагаах ухаан, 2013, 2(5)
HPLC DETERMINATION OF LUTEOLIN AND APIGENIN IN MONGOLIAN MEDICINE SCABIOSA COMOSA FISCH FlOS
( Тойм өгүүлэл )
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Introduction: Scabiosa comosa Fisch flos which was recorded in Encyclopedia of
Mongolian Medicine is commonly used in clinic. It has efficacy of febrifuge and
eliminating “XieRi”. Scabiosa comosa Fisch flos is primarily used for the treatment of
hepatic warm, liver injury poisoning and fleshless disease. However, it is hard to assure
the quality of the medicine because there is no systemic qualitative standard for its
manufacture now. According to the reference, luteolin and apigenin in Mongolian
Medicine Scabiosa comosa Fisch flos was determined by HPLC in this paper. Results
showed that the method was advanced and reliable, so it provided scientific basis for
the establishment of quality control of the medicine.
Objective: To establish the standard of quality control of Mongolian medicine
Scabiosa comosa Fisch flos.
Methods: Luteolin and apigenin in Mongolian Medicine Scabiosa comosa Fisch flos
from different origin was determined by HPLC. The column was Dimaonsil-C18
(250mm ×4.6mm，5μm), column temperature was 30 ℃, the flow phase was
acetonitrile-0.4% phosphoric acid in water (34:66) and the detection wavelength was
350nm. The flow rate: 1.0 ml/min, sample: 10 μl.
Results: The linear ranges of luteolin was 66 ~ 396 ng，r=0.9998, apigenin was 93 ~
558 ng，r=0.9996. The average recovery rate of luteolin was 100.96% (RSD=1.42%),
apigenin was 101.67% (RSD=1.81%).
Conclusion: The method was simple, accurate, reliable and reproducible. It provided a
basis for quality control of Mongolian medicine Scabiosa comosa Fisch flos.
Нийтлэлийн нээгдсэн тоо: 388
