Dylenova E.P.

The pharmacognostic analysis of Artemisia frigida Willd. (from Buryatia, Zabaikailsky krai and Qinghai-Tibet plateau) was studied in this research. Macro- and microscopic characteristics of three samples were mostly the same. Purity tests and identification of main biologically active substances in A. frigida Willd were carried out using standard identity tests. The essential oils were obtained by hydrodistillation method, qualitative and quantitative composition was studied by GC-MS. The main components of the essential oils in three samples are camphor, 1,8 – cineol, endo-borneol and borneol, terpinen-4- ol and others.
Keywords: Artemisia frigida Willd., pharmacognostic analysis, essential oils, chemical composition, monoterpenes, sesquiterpenes. Artemisia frigida Willd. is a valuable medicinal plant which is widely spread on the territory of Republic of Buryatia. It is also used in a Tibetan medicine for the treatment of injuries, tuberculosis, deficiency disease and neurological disturbance [1]. Pharmacological activity is associated with the presence of different biologically active substances, especially, essential oils, which demonstrate anti-bacterial, antifungal and anthelmintic activities [2]. The aim of this work is to make a Pharmacognostic analysis of herba Artemisiae frigidae which was collected on the territory of Russia and China. To achieve this goal we should make the following tasks: 1) to do macro- and microscopic analysis; 2) to make purity tests of plant materials; 3) to identify the presence of main biologically active substances; 4) to make a qualitative composition and chemical assay of essential oils by gas chromatography mass spectrometry (GC-MS). For the research we used herba Artemisiae frigidae which was collected in 2014, in such regions like Ivolginsky region (Russia), Zabaikailsky krai (Russia) and Qinghai-Tibet plateau (China) in a flowering stage. Purity tests and the presence of main biologically active substances were identified by using standard identity tests [3,4].The essential oil from the whole aerial part of Artemisia frigida was obtained by hydrodistillation method and was analyzed by gas chromatography–mass spectrometry (GC– MS) to determine its chemical composition and assay [5,6]. Macroscopic analysis revealed that plant material is presented by numerous caules with
a high around 8-40 sm, woody roots. Lower part of leaves are gray colored and on the top they are light green. Flower anthodes are large, spherical, droop and combined in a raceme and has characteristic aromatic odour. Microscopic analysis revealed that the main diagnostic features in 3 samples are the same: flexuous cells of the top epidermis, abaxial and adaxial stoma (anomocytic stomata), sword-like fuzzes and internal secretion glands on two sides of leaves [7]. Also, we made purity tests and identified such characteristics as moisture, total ash, 10% chlorohydric acid-insoluble ash, and we found out that 70% ethanol as a solvent is more suitable for the extraction. In this material we found out the presence of polysaccharides, essential oils, fatty acids, tannins and flavonoids using standard identity tests [8]. According to the quantitative analysis of essential oils in three samples of herba Artemisiae frigidae, made by gas chromatography mass spectrometry (GC-MS), it contains 1,00% of light green essential oil in the raw material from Zabaikailsky krai (Russia). The major components of this essential oil were presented by sesquiterpenes (50.33%), such as germacrene D, caryophyllene, α-zingiberene
and bicyclogermacrene; among monoterpenes - camphor, 1,8-cineol, endo-borneol, terpinen-4- ol, α-terpineol. Chemical assay of the essential oil of A.frigida collected in Buryatia (Russia) is around 0,97%. There were detected about 63 components in this essential oil and, mostly, they are monoterpenes (87.67%), such as camphor, 1,8 – cineol, endo-borneol, myrtenol, α-terpineol 35 МОНГОЛЫН ЭМ ЗҮЙ, ЭМ СУДЛАЛ СЭТГҮҮЛacetate. What concerns the raw material from Qinghai-Tibet plateau (China), it contains 0,67% of essential oils. The major components were presented by monoterpenes like in Buryatian sample (93.85%): camphor, 1,8 – cineol, borneol, terpinen-4-ol and bornyl acetate. Thus, the most chemical assay of the essential oil of A.frigida was from the raw material collected in Zabaikailsky krai (Russia) – 1,00%. But the macro components of the essential oils in three samples are presented by monoterpenes, like camphor, 1,8 – cineol, endo-borneol and borneol, terpinen-4-ol and others. Thus, pharmacognostic analysis of three samples of herba Artemisiae frigidae revealed that macro- and microscopic characteristics are mostly the same. And purity tests and identification of main biologically active substances in A. frigida Willd has been carried out using standard identity tests. The quantitative composition of essential oils is different depending on growing conditions, but the chemical composition of the main components is constant and presented, mostly, by monoterpenes. So this research shows that Artemisia frigida Willd. can be used as a valuable raw material for the medicine.
References:
1. Batorova S.M., Ubasheev I.O. Medical herbs of Zabaikalye, using in Tibetan medicine for wound healing // Resources of Zabaikalyan plant formation and its using. - Ulan-Ude, 1991. P. 169-182.
2. Mikhailova T.N. Antimicrobial activity of essential oils of some species of Artemisia in Siberian flora // some aspects of pharmacognosy and cultivated plants of Siberia. – Tomsk, 1969. P.32-39.
3. State Pharmacopoeia of the USSR. Ed. XI (part 2). Common methods of analysis. Medical plant material / USSR Ministry of Health. - 11th ed., Ext. - Moscow: Medicine, 1990. - 400p.
4. Grinkevich N.I., Safronich L.N. Chemical analysis of medical plants. -Moscow: Higher school, 1983.-176p.
5. Chemical composition of essential oils of Artemisia frigid Willd. from Buryatian flora / S.V. Zhigzhitzhapova and others // Vestnik of Buryat State University. – 2013. - №3. P.71-74.
6. Chemical composition of essential oils of Artemisia frigid Willd., collected in Zabaikalye / N.V. Bodoev and others // Chemistry of plant materials. – 2000. – №3. P.41-44.
7. Macroscopic, microscopic and phytochemical analysis of medicinal plants: teaching aids / S.V. Zhigzhitzhapova, T.E. Randalova, L.D. Radnaeva - Ulan -Ude: Publishing Buryat Scientific Center. - 2014. 93 p.
8. Plant resources of USSR. Flowering plants, its chemical composition, using. Genus Asteraceae. St.Petersburg: Nauka, 1993. 352 P

The pharmacognostic analysis of Artemisia frigida Willd. (from Buryatia, Zabaikailsky krai and Qinghai-Tibet plateau) was studied in this research. Macro- and microscopic characteristics of three samples were mostly the same. Purity tests and identification of main biologically active substances in A. frigida Willd were carried out using standard identity tests. The essential oils were obtained by hydrodistillation method, qualitative and quantitative composition was studied by GC-MS. The main components of the essential oils in three samples are camphor, 1,8 – cineol, endo-borneol and borneol, terpinen-4- ol and others.
Keywords: Artemisia frigida Willd., pharmacognostic analysis, essential oils, chemical composition, monoterpenes, sesquiterpenes. Artemisia frigida Willd. is a valuable medicinal plant which is widely spread on the territory of Republic of Buryatia. It is also used in a Tibetan medicine for the treatment of injuries, tuberculosis, deficiency disease and neurological disturbance [1]. Pharmacological activity is associated with the presence of different biologically active substances, especially, essential oils, which demonstrate anti-bacterial, antifungal and anthelmintic activities [2]. The aim of this work is to make a Pharmacognostic analysis of herba Artemisiae frigidae which was collected on the territory of Russia and China. To achieve this goal we should make the following tasks: 1) to do macro- and microscopic analysis; 2) to make purity tests of plant materials; 3) to identify the presence of main biologically active substances; 4) to make a qualitative composition and chemical assay of essential oils by gas chromatography mass spectrometry (GC-MS). For the research we used herba Artemisiae frigidae which was collected in 2014, in such regions like Ivolginsky region (Russia), Zabaikailsky krai (Russia) and Qinghai-Tibet plateau (China) in a flowering stage. Purity tests and the presence of main biologically active substances were identified by using standard identity tests [3,4].The essential oil from the whole aerial part of Artemisia frigida was obtained by hydrodistillation method and was analyzed by gas chromatography–mass spectrometry (GC– MS) to determine its chemical composition and assay [5,6]. Macroscopic analysis revealed that plant material is presented by numerous caules with
a high around 8-40 sm, woody roots. Lower part of leaves are gray colored and on the top they are light green. Flower anthodes are large, spherical, droop and combined in a raceme and has characteristic aromatic odour. Microscopic analysis revealed that the main diagnostic features in 3 samples are the same: flexuous cells of the top epidermis, abaxial and adaxial stoma (anomocytic stomata), sword-like fuzzes and internal secretion glands on two sides of leaves [7]. Also, we made purity tests and identified such characteristics as moisture, total ash, 10% chlorohydric acid-insoluble ash, and we found out that 70% ethanol as a solvent is more suitable for the extraction. In this material we found out the presence of polysaccharides, essential oils, fatty acids, tannins and flavonoids using standard identity tests [8]. According to the quantitative analysis of essential oils in three samples of herba Artemisiae frigidae, made by gas chromatography mass spectrometry (GC-MS), it contains 1,00% of light green essential oil in the raw material from Zabaikailsky krai (Russia). The major components of this essential oil were presented by sesquiterpenes (50.33%), such as germacrene D, caryophyllene, α-zingiberene
and bicyclogermacrene; among monoterpenes - camphor, 1,8-cineol, endo-borneol, terpinen-4- ol, α-terpineol. Chemical assay of the essential oil of A.frigida collected in Buryatia (Russia) is around 0,97%. There were detected about 63 components in this essential oil and, mostly, they are monoterpenes (87.67%), such as camphor, 1,8 – cineol, endo-borneol, myrtenol, α-terpineol 35 МОНГОЛЫН ЭМ ЗҮЙ, ЭМ СУДЛАЛ СЭТГҮҮЛacetate. What concerns the raw material from Qinghai-Tibet plateau (China), it contains 0,67% of essential oils. The major components were presented by monoterpenes like in Buryatian sample (93.85%): camphor, 1,8 – cineol, borneol, terpinen-4-ol and bornyl acetate. Thus, the most chemical assay of the essential oil of A.frigida was from the raw material collected in Zabaikailsky krai (Russia) – 1,00%. But the macro components of the essential oils in three samples are presented by monoterpenes, like camphor, 1,8 – cineol, endo-borneol and borneol, terpinen-4-ol and others. Thus, pharmacognostic analysis of three samples of herba Artemisiae frigidae revealed that macro- and microscopic characteristics are mostly the same. And purity tests and identification of main biologically active substances in A. frigida Willd has been carried out using standard identity tests. The quantitative composition of essential oils is different depending on growing conditions, but the chemical composition of the main components is constant and presented, mostly, by monoterpenes. So this research shows that Artemisia frigida Willd. can be used as a valuable raw material for the medicine.
References:
1. Batorova S.M., Ubasheev I.O. Medical herbs of Zabaikalye, using in Tibetan medicine for wound healing // Resources of Zabaikalyan plant formation and its using. - Ulan-Ude, 1991. P. 169-182.
2. Mikhailova T.N. Antimicrobial activity of essential oils of some species of Artemisia in Siberian flora // some aspects of pharmacognosy and cultivated plants of Siberia. – Tomsk, 1969. P.32-39.
3. State Pharmacopoeia of the USSR. Ed. XI (part 2). Common methods of analysis. Medical plant material / USSR Ministry of Health. - 11th ed., Ext. - Moscow: Medicine, 1990. - 400p.
4. Grinkevich N.I., Safronich L.N. Chemical analysis of medical plants. -Moscow: Higher school, 1983.-176p.
5. Chemical composition of essential oils of Artemisia frigid Willd. from Buryatian flora / S.V. Zhigzhitzhapova and others // Vestnik of Buryat State University. – 2013. - №3. P.71-74.
6. Chemical composition of essential oils of Artemisia frigid Willd., collected in Zabaikalye / N.V. Bodoev and others // Chemistry of plant materials. – 2000. – №3. P.41-44.
7. Macroscopic, microscopic and phytochemical analysis of medicinal plants: teaching aids / S.V. Zhigzhitzhapova, T.E. Randalova, L.D. Radnaeva - Ulan -Ude: Publishing Buryat Scientific Center. - 2014. 93 p.
8. Plant resources of USSR. Flowering plants, its chemical composition, using. Genus Asteraceae. St.Petersburg: Nauka, 1993. 352 P