Every year 3-5 million cases have reported during human influenza epidemics and 300 000-500 000 of them die. People who aged over 65 and under 2 year counted as a high risk group to influenza.
When mix red blood cells and influenza virus, it shows Hemaglutination reaction, which is caused by binding of influenza virus surface molecule hemagglutinin with red blood cell (RBC) receptor with syalic acid residues. This reaction is used traditionally as main and effective assay for influenza virus detection.
- To test local strains of A(H1N1), A(H3N2), A(H5N1) and B type influenza virus with human, guinea pig, chicken and horse RBCs
- To choose the most sensitive animal RBC for detection of different influenza viruses
We have used isolates of A/Ulaanbaatar/1885/07 (H1N1), А/Ulaanbaatar/1654/07 (H3N2), B/Ulaanbaatar/1877/07 isolated in the Respiratory Virology Laboratory, National Center for Communicable Diseases, A/Wooper Swan/Mongolia/65/06 (H5N1) isolated in Central Veterinary Laboratory with human 01, horse, chicken and guinea pig RBCs. The subtyping of the Human influenza viruses have been performed with rt-PCR with specific primers.
We used protocol of CDC, USA to isolate human influenza strains in MDCK cell culture, to determine strains with Hemagglutination test.
Human Influenza A(H1N1), A(H3N2) and B type viruses are more agglutinable with to guinea pig RBCs. Avian influenza A(H5N1) virus is more agglutinable with human 01 and horse RBC.
Guinea pig RBCs is the most effective for daily human influenza virus detection procedures and using horse and guinea pig RBC together is reasonable for avian influenza suspected cases.
