1 School of Traditional Medicine, HSUM
2 Clinical and Pharmacological Institute of Traditional Mongolian Medicine, National University of Inner Mongolia
3 Centre of Research and Training, School of Health Technology, HSUM
Өгүүллийн PDF-ийг татах
The aim of this study was to examine the effects of calcite compound on bone mineral contents and bone markers of rat model of osteoporosis induced by retinoic acid. Sprague-Dawley rats were randomly divided into 4 groups. Retinoic acid was given at concentration of 70 mg/kg for 14 days. Calcite compound and CalciD-Denk were administered at concentrations of 0.5 g/kg and 1.16 g/kg respectively.
Ash weight of vertebral bones and concentrations of Ca and P in the ashes were determined by using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES). Blood plasma levels bone resorpotion marker- Cross-linked C-telopeptide of collagen type 1 (CTx) and Inflammatory- Associated Marker - receptor activator of nuclear factor kappa-B ligand (RANKL) were measured by ELISA.
Ash weight of vertebral bones and Ca concentration in the bone ashes were significantly reduced after administration of retinoic acid (р<0.001) and they were significantly increased by calcite compound (р<0.001). Levels of СТх and RANKL in the blood plasma significantly increased in rats received retinoic acid (р=0.0001). Calcite compound significantly reduced increases in СТх and RANKL induced by retinoic acid (р=0.0001).
Calcite compound can be beneficial for treatment of osteoporosis through enhancing osteoblast differentiation and bone formation.
