1Traditional Medical Science, Technology and Production Corporation of Mongolia
2Health Sciences University of Mongolia,
3National University of Mongolia, School of Chemistry, Chemical Engineering
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Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz., Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine.
Bioactive compounds of Garidi-5 were determined by the Folin–Ciocalteu method. An antioxidant potential of the methanol extract of Garidi-5 was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay. Writhing was induced in mice by an intraperitoneal injection of 0.6 % acetic acid solution (10 ml/kg). Water extract of Garidi-5 was given orally at concentrations of 20 mg/kg, 80 mg/kg and 200 mg/kg. Aspirin was used as a standard medicine (100 mg/kg).
Gallic acid, α-azarone, costunolide, alkaloids and testosterone were detected in Garidi-5. Methanol extract of Garidi-5 showed the highest (95.11%) DPPH radical scavenging activity at concentration of 0.05 µg/ml. Moreover, Garidi-5 significantly reduced the number of writhes induced by acetic acid in mice by 40.4-47.9% suggesting that it has peripheral antinociceptive effect.
Garidi-5 (P < 0.05) inhibited carrageenan induced hind rat paw edema.
Conclusion: Biological activity substances were detected in the Traditional drug Garidi-5. Traditional drug Garidi-5 has low toxicity, anti-inflammatory and analgesic effects.
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