Mongolian Journal of Health Sciences, 2011, 1(8)

Inhibitory effect of S. alopecuroides on in vitro growth, adherence and acid production of mutans streptococci

( Судалгааны өгүүлэл )

Ulamnemekh hulan¹*, Mashbaljir Soyolmaa¹, Tsutomu Shimono²

¹Department of Operative Dentistry, School of Dentistry, Health Sciences University of Mongolia; ²  Department of Behavioral Pediatric Dentistry, Graduate School of Medicine and Dentistry, Okayama University, Japan

 

   Өгүүллийн PDF-ийг татах

Абстракт

Dried, ground roots of Sophora alopecuroides, one of the most popular herbal remedies of Mongolia, were extracted with distilled water and methanol. Four fractions were obtained from methanol extract, and labeled as ME₁, ME₂, ME₃ and ME₄. The extracts of ME₁, ME₃, and ME₄ of S. alopecuroides inhibited the growth of either Streptococcus mutans or Streptococcus sobrinus, while ME₂ and WE possessed no inhibition activity. The extract of ME₄ inhibited the acid production of both strains at low concentrations, followed by the ME₃ and ME₁, and finally,  the WE and ME₂. ME₄ of S. alopecuroides was the strongest inhibitor of the sucrose-dependent adherence of growing cells of S. mutans and S. sobrinus, followed by the ME₃ and ME₁, and then, by the WE and ME₂. At 10mg/ml, ME₂ completely inhibited the adherence of S. sobrinus, while that of S. mutans was inhibited by 82%. The WE of S. alopecuroides completely inhibited the adherence of S. mutans at 10mg/ml, while it inhibited the adherence of S. sobrinus by 83% at the same concentration. 

Ном зүй

1. Bratthal D and Kohler B. Streptococcus mutans serotypes: Some aspects of their identification, distribution, antidenic shifts, and relationship to caries. J Dent Res. 1976; 55 (Spec Iss): C15-C21.
2. Hamada S and Slade HD. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980; 44 (2): 331-384.
3. Van Houte J. Bacterial specificity in the etiology of dental caries. Int Dent J. 1980; 30 (4): 305-326.
4. Hamada S, Koga T and Ooshima T. Virulence factors of Streptococcus mutans and dental caries prevention. J Dent Res. 1984; 83 (3): 407-411.
5. Tanzer JM. On changing the cariogenic chemistry of coronal plaque. J Dent Res. 1989; 68 (Spec Iss): 1576-1587.
6. Tanzer JM. Microbiology of dental caries. In: Contemporary Oral Microbiology and Immunology (Edited by J. Slots and M. A. Taubman), St. Louis, MO: Mosby-Year Book. 1992; pp. 377-424.
7. Hamada S, Horikoshi T, Minami T, Okahashi N and Koga T. Purification and Characterization of cell-associated glucosyltransferase synthesizing water-insoluble glucan from serotype c Streptococcus mutans. J Gen Microbiol. 1989; 135: 335-344.
8. Scheie AA. Modes of action of currently known chemical anti-plaque agents other than chlorhexidine. J Dent Res. 1989; 68 (Spec Iss): 1609-1616.
9. Marsh PD. Microbiological aspects of the chemical control of plaque and gingivitis. J Dent . 1992; 71 (7): 1431-1438.
10. Segal R, Pisanty S, Wormser R, Azaz E and Sela, MN. Anticariogenic activity of licorice and glycyrrhizine I: Inhibition of in vitro plaque formation by Streptococcus mutans. J Pharm science. 1985; 74 (1): 79-81.
11. Katsura H, Tsukiyama R, Suzuki A and Kobayashi M. In vitro antimicrobial activities of bakuchiol against oral microorganisms. Antimicrob Agents and Chemotherapy. 2001; 45 (11): 3009-3013.
12. Farnsworth NR. Ethnopharmacology and drug development. In: Prance, G. T. (Ed.), Ethnobotany and the Search for New Drugs. 1994; Wiley, Chichester (Ciba Foundation Symposium 185): 42-59.
13. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12 (4): 564-582.
14. Rosen S, Elvin-Lewis M, Beck FM and Beck EX. Anticariogenic effects of tea in rats. J Dent Res. 1984; 63 (5): 658-660.
15. Sakanaka S, Kim M, Taniguchi M and Yamamoto T. Antibacterial substances in Japanese green tea extract against Streptococcus mutans, a cariogenic bacterium. Agricult and Biol Chem. 1989; 53 (9): 2307-2311.
16. Hamilton-Miller JMT. Anti-cariogenic properties of tea (Camellia sinensis). J Med Microbiol. 2001; 50: 299-302.
17. Wolinsky LE, Mania S, Nachnani S and Ling S. The inhibiting effect of aqueous Azadirachta indica (Neem) extract upon bacterial properties influencing in vitro plaque formation. J Dent Res. 1996; 75 (2): 816-822.
18. Wolinsky LE, Sote EO. Isolation of natural plaque-inhibiting substances from “Nigerian chewing sticks”. Caries Res. 1984; 18: 216-225.
19. Bhuiyan MM, Nishimura M and Shimono T. Antibacterial effects of the crude Azadirachta indica Neem bark extract on Streptococcus sobrinus. Ped Dent 1997; 7 (1): 61-64.
20. Bhuiyan MM, Nishimura M and Shimono T. Effect of Azadirachta indica Neem bark extract on the acid production and adherence of Streptococcus sobrinus. Ped Dent 1998; 17 (2): 261-268.
21. Tsuchiya H, Sato M, Iimuna M, Yokoyama J, Ohyama M, Tanaka T, Takase I and Namikawa I. Inhibition of the growth of cariogenic bacterial in vitro by plant flavanones. Experientia. 1994; 50: 846-849

 

Нийтлэлийн нээгдсэн тоо: 240