Mongolian Journal of Health Sciences, 2007, 2(4)

Rp-hplc determination of luteolin in mongolian medicina1 plant Scabiosa comosa fisch

( Судалгааны өгүүлэл )

BAI Yu-xia'. Narantuya S.^:, DungerdorjD.² '

College of Mongolian Medicine,

Inner Mongolia University for Nationalities, China

Health Sciences University of Mongolia

 

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Абстракт

The RP-HPLC method of qualitative and quantitative determination of the quality of the medicinal plant Scabiosa comosa Fisch widely used in Mongolian Traditional medicine have been developed. Luteolki has been taken as a standard and HPLC determination were achieved using column C_|8(250*4.6mm), the flow phase CH₃OH-0.4% H,P0₄H₂0(55:45) and the detection wavelength 254nm. The linear ranges of Luteolin was 0.072~0.036ig(r=0.9997). The average recovery rate was 98.19% ( RSD=0.872%). The content of luteolin in Scabiosa comosa provided by the Attached Hospital of Inner Mongolia University for Nationalities and collected from Hanshan mountain and from Hingan, Inner Mongolia were 1.97mg/g, 5.88mg/g and 7.49mg/g respectfully. As well for the Scabiosa comosa the stability test, recovery test and repetition experiment have been done.

Ном зүй

1. Editorial Board of Chinese Medical Encyclopedia. 1982, Chinese Medical Encyclopedia [MJ, Shanghai Scientific& Technical Publishers, Shanghai, Vol. 11,
p.204
2. Editorial Board of Chinese Materia Medica in State Administration of Traditional Chinese Medicine, 2004, Chinese Materia Medica, Shanghai Scientific &
Technical Publishers, Shanghai, p.342
3. BAlYu-xia, WangNai Li, Yaoxin Sheg, 1989, Bioactive compounds of Scabiosa comosa Fisch, Journal of Chinese herbal medicine, Vol.20, No.6, pp.7-8

 

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