Дорно дахины уламжлалт анагаах ухаан, 2012, 2(3)
EFFECT OF DEVA-5 ON BACTERIAL GROWTH IN VITRO
( Тойм өгүүлэл )
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Traditional medicine describes common flu as excess of blood and bile heat combined
with microbes which results infectious heat. Deva-5 is a decoction used which treats
infectious heat. The aim of this study is to analyze components of Deva-5 decoction by
traditional methods. Deva-5 is composed of Gentiana decumbens L., Terminalia
Chebula Retz., Polygonium bistorta L., Momordica cochinchinensis (Lour) and
Chiazospermum erectum Bernh. It has a cool power and dispels infectious heat and
kills microbes. The main component of the decoction is Gentiana decumbens L. It has a
sweat taste and clears heat, kills microbes, and cures typhus.
The effects of water extract of Deva-5 on pathogenic bacteria including Streptococcus
pneumonia, Staphylococus aureus, and Escherichia coli were examined. Plates of
bacteria treated with Deva-5 at 6 different concentrations (1 -30 %) were incubated at
37°C for 24 h and then the diameter of the circle of bacterial growth was measured.
Amoxicillin, nistatin, flukanosol, and ciprofloxacin were used as comparison. Acute
and chronic toxicities of Deva-5 were determined in mice. Deva-5 was given orally to
mice at doses of 200 mg/kg and 500 mg/kg for 28 days. In addition, therapeutic dose of
Deva-5 was also determined in mice.
Deva-5 inhibited growths of Streptococcus pneumonia and Staphylococus aureus at
concentrations of 5 %, 10 %, 20 %, and 30 %. Deva-5 more effectively inhibited
growth of Streptococcus pneumonia than that of Staphylococus aureus. Growth of
Escherichia coli was not inhibited by Deva-5 treatment. The median lethal dose of
(LD50) of Deva-5 was 6.89 g/kg. There were no difference between the control and
Deva-5-treated groups in movements, hair color, food consumption, and the number of
offspring of mice. No histopathological changes were observed in solid organs of
animals treated with Deva-5. Therapeutic doses of Deva-5 were found to be 50-150
mg/kg. These results suggest that Deva-5 has antibacterial effects in vitro and it is safe
in mice.
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