1-Department of Nanobiomedical Science & BK21+ NBM Global Research Center for Regenerative Medicine, Dankook University,Cheonan 330714, South Korea Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330714, South Korea khandmaa21@dankook. ac.kr 976-9189-8383 , 82-10-7183-6336

BACKGROUND

Scaffolds with the capacity to deliver signaling molecules are attractive for bone regeneration. Here, we developed bioactive siloxane–gelatin hybrid scaffolds via a sol gel process containing stromal derived factor-1 (SDF-1) to recruit osteoprogenitor/stem cells. 

METHODS

The process was undertaken under room temperature aqueous conditions, which enabled therapeutic molecules to be effectively incorporated. After the sol-gel reaction and lyophilization process, well crosslinked hybrid scaffolds were obtained with porosities of 80–90%.

RESULTS

Dynamic mechanical analysis of the hybrid scaffolds showed significant improvement in storage modulus values (from 10 to 110 kPa) with increasing siloxane content. Target molecule SDF-1 was loaded and released from the scaffolds, and the effects on the homing of mesenchymal stem cell were studied.

CONCLUSIONS

Results demonstrated significant enhancement in the migration of cells to the SDF-1 loaded scaffolds. Taken together, the developed hybrid scaffolds areconsidered to be useful in loading and delivering signaling molecules such as SDF-1 to recruit osteoprogenitor /mesenchymal stem cells in the bone regeneration process.

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