Idrisov Alim, Almabaev Idris, Almabaeva Aigul

Kazakh National Medical University (The Republic of Kazakhstan) Achievements of modern surgery in treatment of chronic liver pathology is connected with working out of low-invasive methods directed to maximal stimulation of factors of repair regeneration of liver tissue (M. Aliev,    Zh.    Doskaliev,    K.
Omarova).Transplantation of fetal tissue and cells comes to be the most modern and less traumatical in treatment of chronic liver deficiency.
Though methods of transplantation of fetal tissue is available some; shortcomings with them do exist. Transplantation is made by way of intraperitoneal injections or intra-liver by-parts method. Diffusive affection of liver is known to take place under cirrhosis and chronic hepatopathy. Due to that we have began studying a method of even transplantation of fetal hepatocytes. Animals -20 dogs -have shown haemostasia in the result of pulmonectomy. The animals were divided into two groups. In the first group to 10 dogs embryo hepatocytes was injected by way of by-parts method and in the second group even transplantation was made to 10 dogs. To define the extent of 6 liver lobes of the animals dorsal surface length was measured. The lobes length proved to be different. Moreover, the length appeared to be in direct correlation with the animals body weight. The lobes length varied from 9 to 14 cm. The liver was needled subcapsular 1,5 -0,45 cm in depth and 7 -cm in length (average -9,6 -1,2 cm) which is 2 cm less of liver lobes. Not to hurt the liver tissue the syringe has been connected by help of T -joint with water monometer and the pressure in the system syringe -liver tissue was being controlled. Embryo hepatocytes suspension was injected on a scheme: 1 ml per 1 minute. Petal hepatocytes injection was repeated every 3 -4 cm about all dorsal surface of the liver. After implantation was over there was defined viability of embryo hepatocytes which still found in the
syringe. Under by-parts method of injection the amount of fetal hepatocytes appeared to be 18,3 % less and under the offered method -only by 2,1% less. To study the liver morphology of a receiver after implantation histological blocks have been prepared. After 24 hours under by-parts method of injection of fetal hepatocytes in the liver tissue of the receiver there have been noticed large hemorrhages with rupture of walls sinusoidal capillaries. Fetal hepatocytes are located irregularly. With fetal hepatocytes implanted by the offered method rupture of liver tissue was not noticed. There noticed separate spot hemorrhages at the injection places. Embryo hepatocytes are oriented to each other and to liver trabecules of the receiver. After 3 months of fetal hepatocytes implantation by-parts method there have been watched active proliferation of the liver parenchyma. But these processes were of a casual character. During this period under fetal hepatocytes implantation by-parts method there have been noticed active mitosis of lever tissue in all lobes of the liver. At the injection place lobes formation with hemocirculation restoration came to an end. At remote from fetal hepatocytes implantation places tissue there have been watched formation of liver trabeculas. Herewith liver cells mitosis was more expressive in the first zone of acinus.
Conclusions:
1.    Fetal hepatocytes injection by-parts method brings about vast areas of hemorrhages in the liver tissue of the receiver. Proliferation of liver cells is of a casual character.
2.    Fetal hepatocytes implantation by even method about full dorsal liver surface promotes repair regeneration in the receiver's liver.