Sisabekov K. E., Dosaev T. M. Kazakh State Medical University, the Republic of Kazakhstan, Almaty

At present the investigation of functional morphology of nerve apparatus, the content of biogenic amines and neuromediators in central and peripheral organs of immunogenesis at norm and pathology are of special interest (Struchko G. U., 1988). In this connection we made it our aim to study hystochemically noradrenergic and cholinergic innervation of thymus and spleen under antigen stimulation. With that purpose male rats weighing 280 - 350 gr aged 6-7 months were once injected hypodermically into right back pad 0,2 ml of 10 % suspension of thrice washed sheep erythrocytes. Control animals were injected with 0,2 ml sodium chloride solution. There were used 24 animals, 6 of them were used as control. The rats were decapitated at the third, fifth and seventh day after antigen injection because at that period peak of the highest growth of antibody titres in blood was observed ( Aznauiyan A. V., Akopdjanyan A. S., 1983; Toropkova T. V., 1898).The material was frozen in liquid nitrogen, sections were made by creostat 15 mkm thick. For revealing of noradrenergic nerve structures the sections were incubated in 2% solution of neutral formalin and fixed in parafine. The sections were painted by hematoxilinaozine and azur-2 and aozine. Specific fluorescence of catelohamines in noradrenergic nerve fibers was watched by means of microscope "Lumam 1-3", cholinergic innervation and parafine sections with common histochemical paint were watched by means of microscope MBI-15 and LOMO P-l. Density of distribution of nerve structures and intensity of reaction at acetilholinesterasa positive nerve structures were defined by means of cytophotometer ( probe diameter is being 15) in agreed units. The measurements were made in 25 fields of vision in control and each stage of the experiment.

 

Analyzing the thymus preparations at sight there was observed decrease of intensity of fluorescence noradrenergic nerve fibers both perivascular and parenhimatose plexus with animals of experimental group. Intensity of

 

fluorescence was defined not only by less than control quantities of varicoses along nerve fibers but by considerably less quantities of terminal branches into parenhime of the organ from the fibers of perivascular plexus.

 

The changes of cholinergic intraorgan nerve apparatus of animals of control group in comparison with noradrenergic are less marked. The data of qualitative treatment - morphometry and citophotometry showed that as antibody titre grows under antigenic stimulation, density of distribution of noradrenergic fibers comes down and approaches minimum at 5-th day, that is at the moment of the highest titre of antibodies.

 

Cholinergic innervation stayed relatively stable under antigenic stimulation and was less subject to variations growing only at peak of the growth of antibody titres.

 

In spleen analyses of morphometry data as also visual analyses of preparations showed that with increase of antibody titre under antigenic stimulation there take place true decrease of density of distribution of noradrenergic structures in all parts of interorgan vascular channel of spleen but especially in perivascular plexus of central arteries, to be exact at places of localization of lymph nods. Density of distribution of noradrenergic nerve structures also as in thymus was subject to less variations but at the moment at peak of antibody titres there was noticed some increase in all parts of interorgan vascular channel. But the most essential dynamics was characteristic for nerve apparatus of central arteries which revealed more than twice excess of mediator syntheses in cholinergic nerve apparatus of central arteries at peak of antibody titre under antigenic stimulation in comparison with control.

 

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