1Уламжлалт Анагаах Ухаан, Технологийн Хүрээлэн, 2АШУҮИС-АУС-Уламжлалт Анагаах Ухааны Институт
Introduction
Alcohol (EtOH) use disorders (AUDs) represent a substantial public health problem worldwide. Over 76 million people present with AUDs 2.5 million deaths were attributed to alcohol (World Health Organization, 2010). Channadshelshi has been used for treatment of alcohol-related, liver disease and intoxication in traditional medicine.
Goal
To determine effect of channadshelshi on voluntary alcoholic wistar rats.
Material and Methods
To examine acute toxicity of Channadselshi were used V.P.Prozorovsky express method (1978) and OECD (2001). Voluntary EtoH consumption measurement (two-bottle choice, intermittent access to EtOH paradigm in wistar rats). Water and 20% ethanol were presented in 200 mlgraduated plastic cylinders with stainlesssteel drinking spouts.The experiment period was 10 weeks total. EtOH consumption was expressed as grams of EtOH consumed per kilogram of body weight/day.
Elevated plus maze. Anxiety associated with EtoH withdrawalwas measured on EPM The maze waselevated 1 m above the floor and contained four 50 cm long, 10 cmwidearms arranged at right angles. The closed arms had opaque walls 30cm high, extending the length of the arm. At the time of the test, eachanimal was placed in the center of the maze facing an open arm andallowed to explore for a 5 min session. During this 5 min test session, theanimal’s number of arm entries and time spent in each arm per entry was recorded on a camera.
Pentylenetetrazol (PTZ) 100 mg/kg dose used in this study was determined as the dose that induced seizures and protection against mortality in mice The experimental protocol was approved by the Ethics Committee of the MNUMS. (№ 14-11/1À)
Results
LD50 of Channadselshi extract was found to be LD50=2.58 (2.1-3.2) gr/kg by V.P.Prozorovsky express method (1978). Channadselshi was no acute toxic by OECD (2001).
Ten and four weeks oral administration of Channadselshi (200 mg/kg) significantly (P<0.01) decreased the consumption of ethanol and significantly (P<0.01) increased time spent in open arm (EPM) in voluntary alcoholic wistar rat compare to control.
The time (sec) of latency the generalized convulsion were significantly (P<0.01) increased of Channadselshi compare to control. The percent of protection against mortality were 80% in PTZ induced seizure in mice.
Conclusions:
- Our study showed that Channadselshi hasreduced consumption of ethanol in voluntary alcoholic wistar rat.
- Channadselshi has central nervous system protection effect against PTZ induced seizure in mice.
Key words: Ethanol consumption, Elevated plus maze, Pentylenetetrazol, Seizure
Pp. 54-60, Table 1, Figures 4, References14
